MACHEREY-NAGEL, the manufacturer of NUCLEOSIL® HPLC phases and one of the leading companies for chromatography products, presents its Reversed Phase Application Guide.
The 160-page publication includes an introduction to the theoretical aspects of HPLC, a lot of information about modern stationary phases, retention, selectivity and mobile phases.
Aside 150 up-to-date and relevant applications the special chapters Do's and Don'ts and trouble-shooting provide a lot of helpful hints and tips for a successful chromatography. A special section explains basic aspects and demands of preparative HPLC.
The applications are divided in the fields of:
Biological and natural compounds
Professional Solutions for HPLC
OPTIMA Capillary Columns
Several different surface modifications based on NUCLEODUR® silica have been developed over the last years providing a full range of specified HPLC phases and an ideal tool for every separation:
NUCLEODUR® C18 Gravity and C8 Gravity
NUCLEODUR® C18 Isis
NUCLEODUR® C18 Pyramid
NUCLEODUR® Sphinx RP
NUCLEODUR® C18 HTec
NUCLEODUR® C18 ec and C8 ec
NUCLEODUR® CN and CN-RP
NUCLEODUR® NH2 and NH2-RP
Ultrafast separations beyond high pressure driven UHPLC Core-shell silica
NUCLEOSHELL® core-shell particle technology from MACHEREY-NAGEL is an alternate route to gain highest column efficiency and resolution at almost the same short run time but with much lower back pressure.
The program of NUCLEOSHELL® surface modifications now comprises the following phases: